COMPREHENSIVE EVALUATION OF DROUGHT RESISTANCE IN WOODY PLANT SPECIES OF THE FAMILY RUTACEAE JUSS. IN THE ARBORETUM OF THE M. M. HRYSHKO NATIONAL BOTANICAL GARDEN OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE
DOI:
https://doi.org/10.31861/biosystems2025.03.397Keywords:
drought tolerance, water regime, water deficit, turgor maintenance capacityAbstract
An assessment of complex summer drought tolerance was conducted via field and laboratory comparison to identify interspecies and sex-related differences in woody species of the family Rutaceae Juss. (Tetradium daniellii (Benn.) T.G.Hartley ⚥; Phellodendron amurense Rupr. ♂, ♀; and Ptelea trifoliata L. ♂, ♀). The research covered both 2023 and 2024 three-month vegetation periods; conducted in the dendrological collection at M. M. Gryshko National Botanical Garden, NAS of Ukraine (Kyiv). Findings revealed pronounced interspecies and sex-related differences via leaf water regime indicators: water content, turgor maintenance, water deficit, and water-holding capacity. Results indicate that the 2-year field data and 1-year laboratory data were consistent, except for P. trifoliata, where no field differences between sexes were recorded. However, male plants had the least water loss and highest turgor maintenance, especially during the first two months of summer. T. daniellii exhibited a stable stress response except short-term decreases in July. Analysis of water deficit fluctuations in Ph. amurense revealed distinct stress response strategies. Female plants were characterized by an acute but short-term reaction to peak drought, followed by rapid recovery. Male individuals, however, demonstrated a more chronic type of stress, with a lesser ability to restore water balance after the dry period. Statistical analysis of plant water status used Welch’s test (p < 0.05), identifying interspecies and sex-related differences. Data revealed P. trifoliata and T. daniellii as strong summer drought-resistant taxa.
References
1. Vysotska, N. Yu. (2023). Drought resistance of ten poplar clones in eastern Ukraine. Forestry and Forest Melioration, (142), 47–58. https://doi.org/10.33220/1026-3365.142.2023.47
2. Didenko, S. Ya., Nehrash, Yu. M., Rak, O. O., Kushnir, N. V., & Shindler, O. I. (2022). Conservation of plant diversity in selected regions of Eurasia [Zberezhennia riznomanitnosti roslyn okremykh rehioniv Yevrazii]. In N. V. Zaimenko, D. B. Rakhmetov, M. B. Haponenko, & M. I. Shumyk (Eds.), Fundamentalni ta prykladni aspekty introduktsii i zberezhennia roslyn u Natsionalnomu botanichnomu sadu im. M. M. Hryshka NAN Ukrainy: monohrafiia (p. 73). Kyiv: Lira-K. (in Ukrainian)
3. Krugliak, Yu. M. (2010). Water regime and drought resistance of leaves of species, forms, and hybrids of the genus Salix L. Plant introduction, (1), 85–89. https://doi.org/10.5281/zenodo.2553623
4. Mamchur, V. V. (2019). Assessment of drought resistance of Ailanthus altissima (Mill.) in conditions of the Right-Bank Forest-Steppe and Steppe of Ukraine. [Otsinka posukhostiikosti Ailanthus altissima (Mill.) v umovakh Pravoberezhnoho Lisostepu i Stepu Ukrainy]. Scientific Bulletin of the Ukrainian National Forestry University, 29(7), 31–33. https://doi.org/10.15421/40290706
5. Mezhenskyi, V. M. (2007). Unification of rating scales used in the introduction of woody plants. [Unifikuvannia shkal otsinok, shcho zastosovuiutsia pry introduktsii derevnykh roslyn]. Plant introduction, (4), 26–37. (in Ukrainian)
6. Meteopost. (2025). Weather archive. Retrieved August 25, 2025, from https://meteopost.com/weather/archive
7. Parashchuk, O. A. (2025). Tetradium Lour. (Rutaceae Juss.) in dendrological collections and the potential of the genus for cultivation. Bioriznomanittia, ekolohiia ta eksperymentalna biolohiia, 27(1), 6–21. https://doi.org/10.34142/2708-5848.2025.27.1.01
8. Sliusar, S. I., & Kuznietsov, S. I. (2008). Introduction of taxodiaceous species (Taxodiaceae F. W. Neger) in the Forest-Steppe of Ukraine [Introduktsiia taksodiievykh (Taxodiaceae F. W. Neger) v Lisostepu Ukrainy]. Kyiv: Natsionalnyi aviatsiinyi universytet, 83–87. (in Ukrainian).
9. Tsentralna Heofizychna Observatoriia im. B. Sreznevskoho. (2025). Weather summary. Retrieved July 19, 2025, from http://www.cgo-sreznevskyi.kyiv.ua/uk/diialnist/21213/9-pidsumki-shchedrogo-na-rekordi-chervnya-u-stolitsi.
10. Chen, L., Zhang, S., Zhao, H., Korpelainen, H., & Li, C. (2010). Sex-related adaptive responses to interaction of drought and salinity in Populus yunnanensis. Plant, Cell & Environment, 33(10), 1767–1778. https://doi.org/10.1111/j.1365-3040.2010.02182.x
11. Dietz, K.-.-J., Zörb, C. and Geilfus, C.-.-M. (2021), Drought and crop yield. Plant Biol J, 23: 881-893. https://doi.org/10.1111/plb.13304.
12. Han, Y., Wang, L., Zhang, X., Korpelainen, H., & Li, C. (2013). Sexual differences in photosynthetic activity, ultrastructure and phytoremediation potential of Populus cathayana exposed to lead and drought. Tree Physiology, 33(10), 1043–1060. https://doi.org/10.1093/treephys/tpt086
13. He, F., Wu, Z., Zhao, Z., et al. (2022). Drought stress drives sex-specific differences in plant resistance. Science of the Total Environment, 845, Article 157171. https://doi.org/10.1016/j.scitotenv.2022.157171
14. Juvany, M., & Munné-Bosch, S. (2015). Sex-related differences in stress tolerance in dioecious plants: A critical appraisal in a physiological context. Journal of Experimental Botany, 66(20), 6083–6092. https://doi.org/10.1093/jxb/erv343
15. Kim, M.-S., Kim, S.-H., Song, J.-H., & Kim, H.-S. (2014). Analysis of secreted nectar volume, sugar and amino acid content in male and female flower of Evodia daniellii Hemsl. Journal of Korean Forest Society, 103(1), 43–50. https://doi.org/10.14578/JKFS.2014.103.1.43
16. Nour, M., Neigh, S., Akmalia, R., & López, S. (2024). Drought responses and adaptation in plants differing in life-form. Frontiers in Ecology and Evolution, 12, Article 1452427. https://doi.org/10.3389/fevo.2024.1452427
17. Nuzhyna, N., Ivanova, I., Hrytsak, L., & Drobyk, N. (2022). Drought-resistant species of trees and bushes are an important link for reducing the negative effects of "urban heat islands". Scientific Issue of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology, 82(3), 37–43. https://doi.org/10.25128/2078-2357.22.3.6
18. Pokhylchenko, O., Gorelov, O., Dovhalyuk, N., Kozubenko, T., Kruglyak, Y., & Paraschuk, O. (2024). Phenological observations in dendrological collections: Motivation for the use of the universal coding “BBCH scale”. Journal of Native and Alien Plant Studies, (20), 176–197. https://doi.org/10.37555/2707-3114.20.2024.318688
19. Statology. (2020, December 20). Welch’s t-test: When to use it + examples. Retrieved from https://www.statology.org/welchs-t-test/
20. Yang, X., Lu, M., Wang, Y., Wang, Y., Liu, Z., & Chen, S. (2021). Response mechanism of plants to drought stress. Horticulturae, 7(3), Article 50. https://doi.org/10.3390/horticulturae7030050
