INDICATORS OF AMMONIA METABOLISM IN RATS UNDER THE CONDITIONS OF TOXIC INJURY AGAINST THE BACKGROUND OF A DIETARY PROTEIN DEFICIENCY
DOI:
https://doi.org/10.31861/biosystems2024.02.201Keywords:
acetaminophen, toxic damage, ammonia, hyperammonemiaAbstract
The aim of the work was to study the main indicators of ammonia metabolism, in particular, the content of residual nitrogen, glutamine and ammonia nitrogen in blood serum under the conditions of administration of toxic doses of acetaminophen and different regimes of protein nutrition. Determination of residual nitrogen was carried out by a method based on the interaction of nitrogen from all nitrogen-containing substances in blood serum with sulfuric acid to form ammonium sulfate, which forms a yellow-orange compound with Nessler's reagent, the color intensity is recorded at 440-450 nm. The concentration of glutamine in blood serum was determined using an automatic amino acid analyzer T-339. It is shown that a significant increase in the level of residual nitrogen is observed only in animals with acetaminophen toxic damage, which were on full nutrition. At the same time, in rats under conditions of protein deficiency in the diet or with toxic damage against the background of alimentary protein deficiency, the value of this indicator does not change reliably compared to the control. At the same time, our studies showed an increase in the content of glutamine in animals under the conditions of administration of toxic doses of acetaminophen against the background of dietary protein deficiency. The obtained results probably indicate a redistribution between individual components of residual nitrogen in the NPR/TU group under conditions of preservation of its total content. Determination of ammonia nitrogen content showed that a significant decrease in the level of this indicator is observed in animals that consumed a low-protein diet. Instead, the growth of this indicator is observed in groups of rats that were simulated toxic damage by acetaminophen and toxic damage against the background of alimentary protein deficiency, which indicates the formation of a state of hyperammonemia under the studied conditions. Therefore, under the conditions of toxic damage by acetaminophen in the blood serum, the content of both residual nitrogen and ammonia nitrogen increases, while in animals with acetaminophen intoxication against the background of protein deficiency, only the content of ammonia nitrogen increases and the content of residual nitrogen in the serum remains at the control level. The obtained results indicate that the formation of ammonia increases under the studied conditions, but the process of formation of non-toxic nitrogen-containing metabolites is disturbed
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