SCREENING OF CULTIVATION CONDITIONS FOR TRAMETES HIRSUTA ІВК 1569 FOR ENDOPOLYGALACTURONASE PRODUCTION
DOI:
https://doi.org/10.31861/biosystems2026.01.019Keywords:
biotechnology pectinolytic enzymes, cultivation parameters, nutrient medium composition, enzyme yieldAbstract
The study investigated the conditions for endopolygalacturonase synthesis by the Trametes hirsuta 1569 strain under submerged cultivation. It was established that the maximum enzyme activity was achieved on the 9th day, after which a decrease in activity was observed. Among the studied carbon sources, sugar beet pulp proved to be the most effective, whereas brewer’s spent grain did not induce enzyme synthesis. The optimal concentration of sugar beet pulp was 10 g/dm3, which ensured a high level of activity without a further saturation effect. Maximum enzyme synthesis was observed when urea and ammonium sulfate were used as nitrogen sources, with the optimal concentration of (NH4)2SO4 being 1.0 g/dm3. The most favorable pH value for endopolygalacturonase synthesis was in the range of 5–7, with a maximum at pH 6. A moderate agitation rate (120 rpm) and inoculum size of 5 % ensured maximum enzyme synthesis. The obtained results indicate the feasibility of using lignocellulosic wastes, particularly sugar beet pulp, for endopolygalacturonase biosynthesis and can be used to optimize biotechnological processes.
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