СКРИНІНГ УМОВ КУЛЬТИВУВАННЯ TRAMETES HIRSUTA ІВК 1569 ДЛЯ ОТРИМАННЯ ЕНДОПОЛІГАЛАКТУРОНАЗИ
DOI:
https://doi.org/10.31861/biosystems2026.01.019Ключові слова:
біотехнологія, пектинолітичні ферменти, параметри культивування, склад поживного середовища, вихід ферментуАнотація
У роботі досліджено умови синтезу ендополігалактуронази штамом Trametes hirsuta 1569 при глибинному культивуванні. Встановлено, що максимальна активність ферменту досягалася на 9 добу, після чого спостерігалося її зниження. Серед досліджених джерел вуглецю найбільш ефективним виявився буряковий жом, тоді як пивна дробина не індукувала синтез ферменту. Оптимальна концентрація бурякового жому становила 10 г/дм3, що забезпечувало високий рівень активності без подальшого ефекту насичення. Максимальний синтез ферменту спостерігався при використанні сечовини та сульфату амонію як джерел азоту, причому оптимальна концентрація (NH4)2SO4 становила 1,0 г/дм3. Найсприятливіше для синтезу ендополігалактуронази значення pH середовища знаходилось у межах 5–7 з максимумом за pH 6. Помірна швидкість перемішування (120 об/хв) та внесення 5 % посівного матеріалу забезпечують максимальний синтез ферменту. Отримані результати свідчать про доцільність використання лігноцелюлозних відходів, зокрема бурякового жому, для біосинтезу ендополігалактуронази та можуть бути використані для оптимізації біотехнологічних процесів.
Посилання
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