THE ROLE OF PYRUVATE IN ENERGY TURNOVER OF RATS WITH ALLOXAN DIABETES UNDER THE CONDITIONS OF MELATONIN INJECTIONS
DOI:
https://doi.org/10.31861/biosystems2024.02.215Keywords:
melatonin, alloxan diabetes, rats, pyruvateAbstract
It is known that pyruvate is an intermediate product that links glycolysis and the tricarboxylic acid cycle with the subsequent formation of ATP, and can also be used in the formation of essential amino acids and fatty acids. Melatonin is a hormone widely known as an antioxidant and cytoprotector. Melatonin is a hormone widely known as an antioxidant and cytoprotector. The way that pyruvate concentration changes under conditions of diabetes and melatonin administration is still poorly understood.
Sexually mature purebred white rats were divided into groups: 1) control, 2) control + melatonin 5 mg/kg body weight, 3) alloxandiabetic rats (alloxan monohydrate intraperitoneally at the rate of 170 mg/kg body weight), 4) alloxandiabetic rats receiving melatonin similarly Rats were killed by decapitation under light ether anesthesia on the 19th day from the beginning of the experiment in accordance with established norms for the treatment of experimental animals. The level of pyruvate in the blood plasma was measured according to the standard method. Statistical processing of the research results was carried out using the Student's t-test.
The level of glucose and pyruvate in the blood plasma of diabetiv rats increased on 97% and 172% respectively compared to the intact rats. Administration of melatonin caused a 38% decrease in fasting glucose and a normalization of pyruvate compared to intact rats. It is possible that melatonin has a positive effect on the regulation of pyruvate dehydrogenase gene expression. The introduction of melatonin under the conditions of the physiological norm was affected by an increase in the content of pyruvate in the plasma by 23% in comparison with the indicators of intact animals. Such results indicate the switching of energy reactions in favor of aerobic processes.
It was found that in rats with alloxan diabetes, there was an increase in the level of glucose and pyruvate in the blood. Under the conditions of melanin administration, the studied indicators decreased, probably due to the improvement of glucose entry into the cell and the redirection of pyruvate towards aerobic processes, which are more beneficial for the energy supply of the cell.
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