ASSESSMENT OF CHANGES IN THE BIOCHEMICAL STORE OF STUDENTS' SALIVA DURING PHYSICAL ACTIVITIES OF VARIOUS INTENSITY
DOI:
https://doi.org/10.31861/biosystems2024.02.182Keywords:
pH, lactate, calcium, phosphorus, α-amylase, saliva, physical activityAbstract
The aim of the work was to study changes in saliva pH, lactate content, mineral composition and α-amylase activity during high and moderate intensity physical activity on the body of students with different levels of physical fitness.
The study involved 2 groups of students: Group 1 – 13 people who attended only physical education classes 2 times a week and performed moderate intensity physical exercises; Group 2 – 12 students who, in addition to academic classes, attended a daily athletics section and performed intense physical activity. Saliva was collected at rest and immediately after completing physical activity of varying intensity. The total calcium, inorganic phosphorus, lactate, α-amylase activity and pH levels were determined in the saliva of students using conventional biochemical methods and standard reagent kits. It was found that high-intensity physical activity leads to a significant increase in pH, lactate concentration, total calcium, inorganic phosphorus and α-amylase activity in the saliva of highly trained students, which reflects the body's response to training stress and indicates the adaptive capacity of the body and metabolic needs caused by physical activity.The results obtained can be used to monitor parameters such as pH, lactate concentration, calcium/phosphorus content and α-amylase activity in saliva, which can provide valuable information on the physiological adaptation of the body during physical activity of varying intensity.
References
1. Belskaya, L.V, Sarf, E.A, Kosenok, V.K. (2020). Age and gender characteristics of the biochemical composition of saliva: Correlations with the composition of blood plasma. J Oral Biol Craniofac Res, 10(2), 59–65. https://doi.org/10.1016/j.jobcr.2020.02.004
2. Ferradans, L., Moreira, A., Coto, N., Aoki, M., Dias, R. (2020). Does oral hygiene influence salivary pH, lactate, and IL-1b of basketball players during intense exercise? Int. J. Odontostomat, 14(4), 617-622. https://doi.org/10.4067/S0718-381X2020000400617
3. Franco-Martіnez, L., Tvarijonaviciute, A., Martіnez-Subiela, S., Mаrquez, G., Martіnez, Dіaz N., Cugat, R. (2019). Changes in lactate, ferritin, and uric acid in saliva after repeated explosive effort sequences. J. Sports Med. Phys. Fit., 59(6), 902–909. https://doi.org/10.23736/S0022-4707.18.08792-3
4. Jespersgaard, C., Hajishengallis, G., Russell, M.W., Michalek, S.M. (2002). Identification and characterization of a non immunoglobulin factor in human saliva that inhibits Streptococcus mutans glucosyltransferase. Infect. Immun., 70(3), 1136–1142. https://doi.org/10.1128/iai.70.3.1136-1142.2002
5. Hermann, R., Lay, D., Wahl, P., Roth, W., Petrowski, K. (2019). Effects of psychosocial and physical stress on lactate and anxiety levels. Stress. 22(6), 664–669. https://doi.org/10.1080/10253890.2019.1610743
6. Lombardi, G., Ziemann, E., Banfi, G., Corbetta, S. (2020). Physical Activity-Dependent Regulation of Parathyroid Hormone and Calcium-Phosphorous Metabolism. Int. J. Mol. Sci. 21(1), 53–88. https://doi.org/10.3390/ijms21155388
7. Ntovas, P., Loumprinis, N., Maniatakos, P., Margaritidi, L., Rahiotis, Ch. (2022). The Effects of Physical Exercise on Saliva Composition: A Comprehensive Review. Dent. J. 10(7), 2–16. https://doi.org/10.3390/dj10010007
8. Wunsch, K., Wurst, R., Dawans, B., Strahler, J., Kasten, N., Fuchs, R. (2019). Habitual and acute exercise effects on salivary biomarkers in response to psychosocial stress. Psychoneuroendocrinology. 106(2), 216–225. https://doi.org/10.1016/j.psyneuen.2019.03.015
