THE INTENSITY OF FREE RADICAL PROCESSES IN THE LIVER MITOCHONDRIAL FRACTION UNDER THE CONDITIONS OF TOXIC INJURY AND CORRECTION BY THE 3,4-DIHYDROPYRIMIDINE-2-ONE AMMONIUM DERIVATIVE
DOI:
https://doi.org/10.31861/biosystems2017.02.197Keywords:
the 3,4-dihydropyrimidine-2-one ammonium derivative, superoxide-anion radical, TBA-active products, protein carbonyl derivatives, mitochondrionAbstract
The research deals with the determination of the superoxide-anion radical, TBA-active products and protein carbonyl derivatives content in the protein-deficient rat liver mitochondrial fraction under the conditions of acetaminophen-induced hepatitis and correction by the 3,4-dihydropyrimidine-2-one ammonium derivative. The researches were conducted on white rats of 90-100 g body mass aged 2-2.5 months. There were used rats, which according to the experimental model were separated into the following groups: I – animals receiving full-value semisynthetic ration (C); II – animals receiving low-protein ration (LPR); III – animals with acetaminophen-induced liver injury receiving complete ration (H); IV – animals with acetaminophen-induced liver injury that were previously maintained on semi-synthetic low-protein ration (LPR+H); V-XІІ – animals, which were introduced with the examined 3,4-dihydropyrimidine-2-one ammonium derivative at concentrations of 5; 10; 15; 20; 25; 30; 40; 50 mg/kg of the body weight correspondingly. The examined compound concentrations were introduced orally in 2 hours after acetaminophen exposure during 2 days according to GLP recommendation. The most pronounced antioxidant effect at concentration of 15 mg/kg of the 3,4-dihydropyrimidine-2-one ammonium derivative was established. It is observ d that the introducing of the examined compound in the specified concentration leads to the maximal decrease of superoxideanion radical, TBA-active products and protein carbonyl derivatives content in the rat liver mitochondrial fraction with toxic injury to control values. The established patterns are confirmed by a correlation analysis, which was shown the existence of a close correlation between the concentration of test compound and superoxide-anion radical content. The 3,4-dihydropyrimidine-2-one ammonium derivative is a promising compound for the creation on its basis of an antioxidant agent which is capable to effectively suppression of the intensity of free radical processes in the liver mitochondria under the conditions of toxic injury.
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