THE CHANGES OF ACTIVITY OF SEPARATE CARBOHYDRATE METABOLISM ENZYMES IN BLOOD ERYTHROCYTES OF PREGNANT RATS UNDER VANADIUM CITRATE
DOI:
https://doi.org/10.31861/biosystems2019.01.034Abstract
The data on the effect of vanadium citrate, which is organic, on carbohydrate metabolism and the content of estradiol in the blood of rats, as well as the relationship between these indicators are missing. Therefore, the aim of the work was to study the effect of this compound on the activity of glucose-6-phosphate dehydrogenase, lactate dehydrogenase, estradiol content and to establish correlations between the activity of the studied enzymes and the estradiol content in pregnant female rats under the effect of vanadium compound. The study was carried out on 25 female Wistar rats Rattus norvegicus, divided into five groups: group I - non-pregnant females, group II – pregnant females consuming pure water without additives, groups III, IV, V - females which during the mating and pregnancy periods received the solution of vanadium citrate at the concentrations of 3.75, 15.63 and 62.5 μgV/kg of body weight, respectively. Our studies have shown that glucose-6-phosphate dehydrogenase, lactate dehydrogenase and estradiol content increase in pregnant females of group II compared to group I of non-pregnant animals. The increase in the activity of carbohydrate metabolism enzymes in pregnant females of group ІІ may be due to the activation of the pentosophosphate pathway and the intensification of glycolysis in erythrocytes. In pregnant females, the effect of vanadium citrate resulted in the decrease in the activity of glucose-6-phosphate dehydrogenase and lactate dehydrogenase in the blood of animals in groups IV and V, and the content of estradiol in the animals of all three experimental groups (III, IV, V), compared to group II of pregnant females. It has also been established that under the effect of this compound between glucose-6-phosphate dehydrogenase and lactate dehydrogenase activity in pregnant females there is a strong reverse correlation (r = -0.92), between lactate dehydrogenase activity and estradiol content – a weak correlation (r = -0.42 ), while between the content of estradiol and glucose-6-phosphate dehydrogenase activity there is a strong direct correlation (r = 0.74). The values of glucose-6-phosphate dehydrogenase activity in the animals of group IV, which received the solution of vanadium citrate at the concentration of 15.63 μgV/kg of body weight, was similar to that of the animals in group I. This may indicate the normalizing effect of vanadium citrate on the activity of glucose-6-phosphate dehydrogenase at the indicated concentration, which is probably due to the ability of the vanadium microelement to inhibit the activity of this enzyme. The decrease in lactate dehydrogenase activity in groups IV and V, compared to group II, may also be due to the effect of this compound. Vanadium citrate at the concentration of 15.63 μgV/kg of body weight caused the recovery of the enzyme activity to the values of the activity in group І. At this concentration, the most remarkable positive effect on the enzyme activity of carbohydrate metabolism and the content of estradiol is observed. Our studies allow to consider this vanadium compound as a potential dietary supplement for pregnant women.
References
Bizerea T.O., Dezsi S.G., Marginean O., Stroescu R., Rogobete A., Bizerea-Spiridon O., Ilie C. The link between selenium, oxidative stress and pregnancy induced hypertensive disorders. Clin Lab. 2018;64(10):1593–1610. doi: 10.7754/Clin.Lab.2018.180307.
Burton G.J., Jauniaux E. Placental oxidative stress: from miscarriage to preeclampsia. J Soc Gynecol Investig. 2004;11(6):342–52. doi:10.1016/j.jsgi.2004.03.003.
Вutt A.A., Michaels S., Greer D. Serum LDH level as a clue to the diagnosis of histoplasmosis. AIDS Reader. 2002;12(7):317–321. PMID:1216185.
Catanzerite V.A., Steinberg S.M., Mosley C.A., Landers C.F., Cousins L.M., Schneider J.M. Severe preeclampsia with fulminant and extreme elevation of aspartate aminotransferase and lactate dehydrogenase levels : high risk for maternal death. Am J Perinatol. 1995;12:310–3. doi:10.1055/s-2007-994482.
Chandra A.K., Ghosh R., Chatterjee A. Vanadiuminduced testicular toxicity and its prevention by oral supplementation of zinc sulphate. Toxicology Mechanisms and Methods. 2007;17:175–187. doi:10.1080/15376510601185871.
Crans D.C., Smee J.J., Gaidamauskas E., Yang L. The chemistry and biochemistry of vanadium and the biological activities exerted by vanadium compounds. Chemical Reviews. 2004;104(2):849–902. doi:10.1021/cr020607t.
Golbasi H., Ince O., Golbasi C., Ozer M., Demir M., Yilmaz B. Effect of progesterone/estradiol ratio on pregnancy outcome of patients with high triggerday progesterone levels undergoing gonadotropinreleasing hormone antagonist intracytoplasmic sperm injection cycles: a retrospective cohort study. J Obstet Gynaecol. 2018;3:1–7. doi: 10.1080/01443615.2018.1504204.
Frosali S., Di Simplicio P., Perrone S., Di Giuseppe D., Longini M., Tanganelli D., Buonocore G. Glutathione recycling and antioxidant enzyme activities in erythrocytes of term and preterm newborns at birth. Biol Neonate. 2004;85(3):188–94.
Gulersen M., Brost B.C., Bobrovnikov V., Bornstein E. Acute babesiosis in pregnancy: a novel imitator of hemolysis, levated liver enzymes, and low platelet count syndrome. Obstetrics & Gynecology. 2016, 128(1):197–200. doi: 10.1097/AOG.0000000000001445.
Kioseoglou E., Petanidis S., Gabriel C., Salifoglou A. The chemistry and biology of vanadium compounds in cancer therapeutics. Coordination Chemistry Reviews. 2015; 301-302,87–105. doi: 10.1016/j.ccr.2015.03.010.
Korbecki J., Baranowska-Bosiacka I., Gutowska I., Chlubek D. Biochemical and medical importance of vanadium compounds. Acta Biochimica Polonica. 2012;59(2):195–200.View at Google Scholar · View at Scopus PMID:22693688
Kuliszkiewicz-Janus M., Zimny A. Glucose-6- phosphate dehydrogenase (G6PD) deficiency - a cause of anaemia in pregnant women. Pol Arch Med Wewn. 2003;110(5):1327–33. PMID:16737003
Preet A., Gupta B.L., Yadava P.K., Baquer N.Z. Efficacy of lower doses of vanadium in restoring altered glucose metabolism and antioxidant status in diabetic rat lenses. J Biosci. 2005;30(2):221–30. PMID:15886458
Toescu V., Nuttall S.L., Martin U., Kendall M.J., Dunne F. Oxidative stress and normal pregnancy. Clin Endocrinol (Oxf). 2002;57(5):609–13. PMID:12390334
Vlizlo V.V., Fedoruk R.S, Makar I.A. Laboratory methods of research in biology, animal husbandry and veterinary medicine. Lviv: Navy; 2012. 764 p. (in Ukrainian)
Wang Y.X., Chen H.G., Li X.D., Chen Y.J., Liu C., Feng W., Zeng Q., Wang P., Pan A., Lu W.Q. Concentrations of vanadium in urine and seminal plasma in relation to semen quality parameters, spermatozoa DNA damage and serum hormone levels. Science of the Total Environment. 2018;645:441–448. doi: 10.1016/j.scitotenv.2018.07.137
Zizic M., Zivic M., Maksimovic V., Stanic M., Krizak S., Cvetic Antic T., Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. PLoS ONE. 2014;9(7): e102849. doi:10.1371/journal.pone.0102849
Zwolak I. Vanadium carcinogenic, immunotoxic and neurotoxic effects: a review of in vitro studies. Toxicology Mechanisms and Methods. 2014;24(1):1–12. doi: 10.3109/15376516.2013.843110.
