PROBIOTIC CORRECTION OF DAPHNIA MAGNA MICROBIAL PROFILE USING LACTOBACILLUS CASEI UCM 7280

Authors

  • L. KHUDA Yuriy Fedkovych Chernivtsi National University Author
  • M. SPIVAK Danylo Zabolotny Institute of Microbiology and Virology of NAS of Ukraine Author
  • O. DEMCHENKO Danylo Zabolotny Institute of Microbiology and Virology of NAS of Ukraine Author
  • O. KARUCHERU Yuriy Fedkovych Chernivtsi National University Author
  • O. FRUNZA Yuriy Fedkovych Chernivtsi National University Author
  • O. KHUDYI Yuriy Fedkovych Chernivtsi National University Author

DOI:

https://doi.org/10.31861/biosystems2020.01.003

Keywords:

live feed, probiotics, bioencapsulation, Lactobacillus casei, Daphnia magna

Abstract

The anthibiotics use in aquaculture these days is severely restricted by European standarts of marketable fish products quality. According to this, one of the most efficient alternatives are probiotics. So the search of applicable probiotic microorganisms that can be applied in aquaculture is relevant. On the other side, probiotics introduction into the fish body is usually accomplished in composition with dry feed as a biofilm. But a lot of fish larvae are not capable of consuming dry artificial feed due to numerous reasons. For their transmission for endogenic nourishment from exogenic live feeds are used in aquaculture, which have a higher level of digestibility compared to granulated feed. One more advantage of live feed is that they can be used as vectors to provide the targeted delivery of probiotics into fish body. In this work Lactobacillus casei IMV 7280 was firsly tested for the needs of aquaculture. It is shown that the usage of this probiotic during the growing of carp whitebaits stimulates it growth and leads to unwanted microflora suppresion in water as well as in fish bodies. The optimal schedule of L. сasei UCM 7280 bioencapsulation into live feed with Daphnia magna as an example is designed and suggested. It is shown that bioencapsulation procedure realization does not repress D. magna΄s normal mocrobiome, provides the increase of proteins and lipids level for 1,2 and 1,4 times accordingly and spends up the increasing of density level of fodder organisms.

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Published

2020-06-25

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Section

БІОХІМІЯ, БІОТЕХНОЛОГІЯ, МОЛЕКУЛЯРНА ГЕНЕТИКА