INDICATORS OF RENAL STATUS IN RATS WITH ACETAMINOPHEN-INDUCED TOXIC INJURY UNDER THE ADMINISTRATION OF HERICIUM ALPESTRE MUSHROOM ETHANOLIC EXTRACT
DOI:
https://doi.org/10.31861/biosystems2025.02.223Keywords:
acetaminophen, kidney, Hericium alpestre ethanolic extract, oxidative stress markers, urea, creatinine, histological sectionAbstract
The study is dedicated to determining the indicators of renal status under conditions of acetaminophen intoxication and the administration of Hericium alpestre mushroom ethanolic extract. The research was conducted on four groups of animals: K – control rats; TІ – rats with modeled toxic injury; EGA – rats administered per os a 20% ethanolic extract of Hericium alpestre fruiting bodies at a dose of 200 µg/kg body weight for 10 days; EGA+TІ – rats administered per os the 20% Hericium alpestre extract for 10 days prior to modeling the acetaminophen-induced injury. It was established that acetaminophen overdose leads to impaired renal functional status, evidenced by an over twofold increase in serum urea and an almost threefold increase in creatinine compared to the control group. This was accompanied by the activation of reactive oxygen species (ROS) production in the mitochondria: a threefold increase in superoxide (O2•−) and a 1.5-fold increase in hydroxyl radical (HO•) generation, as well as significant structural-functional damage to mitochondrial biomolecules, confirmed by the increase in TBA-reactive substances (TBARS) and protein carbonyl derivatives.
Conversely, the prior administration of the ethanolic extract of Hericium alpestre leads to a significant decrease in the intensity of ROS formation in kidney mitochondria and the inhibition of oxidative damage reactions in biomolecules, shown by TBARS and carbonyl derivative levels approaching control values. Furthermore, the extract led to an improvement in renal excretory function (reduction of urea and creatinine levels in the blood) and a decrease in the percentage of collapsed glomeruli from over 80% in the TІ group to approximately 20% in the EGA+TІ group. The obtained results indicate that the ethanolic extract of Hericium alpestre effectively mitigates the manifestations of acetaminophen-induced toxic injury, preventing both functional and morphological renal disturbances.
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