ГЕНЕТИЧНІ ДОСЛІДЖЕННЯ ПЕКТИНОЛІТИЧНИХ ФЕРМЕНТІВ БАЗИДІЄВИХ МАКРОМІЦЕТІВ
DOI:
https://doi.org/10.31861/biosystems2025.01.059Ключові слова:
пектинолітичні ферменти, базидієві макроміцети, секвенування, транскриптоміка, CAZyАнотація
У статті представлено огляд сучасних уявлень про генетичну організацію та функціональні особливості пектинолітичних ферментативних систем базидієвих макроміцетів. Проаналізовано основні родини ферментів, залучених до деградації пектинових сполук, зокрема глікозилгідролази (GH), полісахаридліази (PL) та карбогідратні естерази (CE), а також відповідні гени, виявлені у представників родів Agaricus, Armillaria, Flammulina, Laccaria, Lentinula, Pleurotus, Schizophyllum і Trametes. Наведено порівняльну характеристику геномів зазначених грибів за кількісними показниками генів пектиназ, їхньою структурною належністю та потенційною функціональною активністю. Розглянуто методологічні підходи до вивчення пектинолітичного потенціалу, зокрема секвенування, транскриптоміку, біохімічні методи та інструменти порівняльної геноміки. Окреслено перспективи подальших досліджень у контексті біотехнологічного застосування ферментів цієї групи.
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